Prevalencia de la infección por Mycoplasma pneumoniae en pacientes pediátricos con exacerbación aguda del asma / Prevalence of Mycoplasma pneumoniae infection in pediatric patients with acute asthma exacerbation

Introducción. El Mycoplasma pneumoniae puede estar implicado en la exacerbación refractaria del asma, Objetivo. Establecer la prevalencia de la infección por Mycoplasma pneumoniae en pacientes con exacerbación aguda del asma. Material y método. Se realizó un estudio prospectivo, transversal, observacional, caso-control, en pacientes mayores de 2 años y menores de 12. Se determinaron anticuerpos inmunoglobulina M (IgM) para M. pneumoniae por serología por técnica de ensayo por inmunoabsorción ligado a enzima (enzyme-linked immunosorbent assay; ELISA en sus siglas en inglés), utilizando el kit NovaLisa® NovaTec. Se consideró prueba positiva a valores > 11 NTU (NovaTec unidades). El análisis estadístico fue análisis de la varianza (analysis of variance; ANOVA, por sus siglas en inglés) y chi cuadrado con un nivel de significancia de p < 0,05. Resultados. Se estudiaron 180 niños, 130 correspondieron al grupo de niños asmáticos y 50, al grupo control. La IgM específica fue positiva en 60 pacientes, que correspondió al 46,15% de niños asmáticos (p < 0,001). La gravedad de la exacerbación estuvo relacionada directamente con los niveles de IgM (p < 0,001). La tasa de hospitalización fue de 75%, asociada de forma significativa con los niveles de IgM específica (p < 0,001). Conclusión. Nuestros datos sugieren que en los niños con asma aguda, tienen alta prevalencia (46%) de infección por Mycoplasma pneumoniae y estrecha relación entre la exacerbación aguda grave del asma y la infección por Mycoplasma pneumoniae. Estos resultados podrían tener implicaciones terapéuticas orientadas hacia la utilización de antibióticos específicos contra este microorganismo atípico.

Introduction. Mycoplasma pneumoniae may be involved in refractory asthma exacerbation. Objective. To determine the prevalence of Mycoplasma pneumoniae infection in patients with acute asthma exacerbation. Material and method. A prospective, crosssectional, observational, case-control study was carried out in patients older than 2 years old and younger than 12. Immunoglobulin M (IgM) antibodies were serologically determined for M. pneumoniae, using the NovaLisa® NovaTec kit for enzyme-linked immunosorbent assay (ELISA). Test results ≥ 11 NTU (NovaTec units) were regarded as positive. The statistical analysis was performed by means of the analysis of variance (ANOVA) and the χ² test, with a significance level of p < 0.05. Results. One hundred and eighty children were studied, of which 130 had asthma and 50 comprised the control group. Specific IgM was positive for 60 patients, that is 46.15% of the asthmatic children (p < 0.001). The severity of the exacerbation was directly related to IgM levels (p < 0.001). Hospitalization rate was 75%, and it was significantly associated to specific IgM levels (p < 0.001). Conclusion. Our data suggest that children with acute asthma show a high prevalence (46%) of Mycoplasma pneumoniae infection and that there is a close relation between severe acute asthma exacerbation and the presence of Mycoplasma pneumoniae infection. These findings might result in therapeutic implications centered in the use of specific antibiotics to fight this atypical organism.

Application of three uniplex polymerase chain reaction assays for the detection of atypical bacteria in asthmatic patients in Kuwait

Respiratory infections are known to exacerbate wheezing in many asthmatic patients. We aimed to use molecular methods for the fast detection of Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila in respiratory specimens from asthmatic patients in Kuwait. We used uniplex PCR assays to detect the three atypical bacteria in clinical specimens from 235 asthmatic and non-asthmatic patients in Kuwait. A regression analysis was used to identify the risk factors related to the bacterial type. Group comparisons for similarity were conducted and correlation coefficients were calculated using SPSS statistical software. The detection limits using uniplex PCR for C. pneumoniae, L. pneumophila and M. pneumoniae were approximately 1 pg, 2.4 fg and 12 pg of DNA, respectively. M. pneumoniae PCR positivity was more common in asthmatic patients [15%] than in non-asthmatic subjects [9%] [P < 0.05]. A marked difference was observed between patients with acute asthma exacerbation [11%] and patients with chronic [stable] asthma [7%] among Kuwaiti patients; these percentages were 16% for non-Kuwaiti acute asthma patients and 14% for non-Kuwaiti chronic asthma patients [P < 0.201]. There was a weak positive correlation between asthma severity and PCR positivity for M. pneumoniae. The PCR results for C. pneumoniae and L. pneumoniae were found to be statistically insignificant. The results of this study suggest that infection with M. pneumoniae may be related to the exacerbation of asthma symptoms and could possibly be a factor that induces wheezing

Polimorfismo Arg16Gly e Gln27Glu do Gene B2-ADR e a sensibilização fúngica associada ao controle e gravidade da asma / B2-adrenergic receptor gene polymorphism Arg16Gly, Gln27Glu and fungal sensitization associated with the control and severity of asthma

A asma brônquica é uma doença inflamatória crônica das vias aéreas inferiores que resulta da interação entre fatores genéticos e ambientais.Teve como objetivo estudar o polimorfismo Arg16Gly e Gln27Glu do Gene β2-ADR e a sensibilização fúngica associada ao controle e gravidade da asma. Trata-se de um estudo observacional analítico caso controle em portadores de asma, assistidos no “Programa de Assistência ao Paciente Asmático do Hospital Universitário Presidente Dutra” (PAPA-HUPD). Foram incluídos no estudo 83 pacientes com asma brônquica e 46 não asmáticos como controles. Os participantes coletaram duas amostra de 5ml de sangue, para genotipagem do gene β2AR-16 e β2AR-27 por PCR-RFLP e teste de sensibilização Elisa específico para fungos. Os dados de controle foram obtidos através do Teste de Controle da Asma (ACT) e os dados clínicos dos prontuários. As análises de polimorfismo não apresentaram associação com o estado de controle da asma para o gene β2-ADR-16 e β2-ADR-27. Entre asmáticos e não asmáticos foi observado diferença estatisticamente significante (p<0,05) com maior expressão do alelo Glu (74%) entre os não asmáticos. O homozigoto Glu/Glu também foi mais frequentes entre os não asmáticos, sugerindo que o alelo Glu-27 pode estar associado ao risco diminuído para asma. Quanto à sensibilização fúngica, não houve associação significante com os genótipos estudados. O estudo mostrou que, asmáticos graves são mais sensibilizados que não asmáticos e asmáticos moderados. Não houve diferença estatisticamente significante entre controle da asma e sensibilização fúngica, no entanto houve associação significativa com a gravidade. Associações isoladas entre sensibilização fúngica e asma, assim como entre polimorfismo e asma ficou evidente neste estudo...

Asthma is a chronic inflammatory disease of the lower airways, and is a result of the interaction between genetic and environmental factors. Aimed to study polymorphism and Arg16Gly Gln27Glu Gene β2-ADR and fungal sensitization associated with asthma severity and control. It is an observational, analytic case-control study involving asthmatic subjects of the Assistance Program to the Asthmatic Patient - APAP, in the Presidente Dutra University Hospital, Maranhão, Brazil. Eighty-three asthmatic patients and 46 non-asthmatic controls were included in the study. PCR-RFLP genotyping of the β2AR-16 and β2AR-27 genes, and fungal-specific enzyme-linked immunosorbent assay (ELISA) were made in two 5 ml blood samples collected from the participants. Control data were gathered from the Asthma Control Questionnaire (ACQ) and clinical data from the medical records. The analysis of the polymorphism has not demonstrated association between the asthma control status and the β2AR-16 and β2AR-27 genes. There was statistically significant difference (p < 0,05) between asthmatics and non-asthmatics, with higher expression (74%) of the Glu allele in non-asthmatic subjects. The Glu/Glu homozygote was also more frequent between non-asthmatics, and it suggests that the Glu-27 allele might be associated with a lower risk to develop asthma. Regarding fungal sensitization, there wasn't significant association with the studied genotypes. The study demonstrated that severe asthmatics are more sensitized than non-asthmatics and moderate asthmatics. There wasn't statistically significant difference between asthma control and fungal sensitization; nevertheless, there was significant association with the severity level. Isolated associations between fungal sensitization and asthma, as well as associations between polymorphisms and asthma were evident in this study...

Effect of Aspergillus fumigatus sensitization and colonization on lung function and airways inflammation in asthma

The sensitization and exposure to fungal allergens have been reported to be associated with asthma. The importance of Aspergillus fumigatus [AF] sensitization and colonization of the airways in patients with asthma is unclear. To clarify the effect of sensitization and airways colonization of AF on lung function and airways inflammation in asthma. We studied 66 patients with asthma. Patients were classified into two groups according to AF sensitization: [1] AF-sensitized [immediate cutaneous reactivity >3 mm]; and [2] AFnonsensitized. A positive sputum culture for AF confirmed airways colonization by AF. Routine spirometry was performed for all patients. Airways inflammation was assessed by sputum differential inflammatory cell count. Asthma duration was significantly longer in AF-sensitized asthmatics. Significantly higher rates of positive AF-culture were detected in sputum from AF-sensitized asthmatics [63%] in comparison to AF-nonsensitized asthmatics [31%]. FEV1 and FEV1/FVC were more reduced in AF-sensitized asthmatics in comparison to AF-nonsensitized asthmatics. Sputum neutrophils count was significantly higher in AF-sensitized asthmatics in comparison to AF-nonsensitized asthmatics. Sputum eosinophils did not differ between AF-sensitized and AF-nonsensitized asthma groups, concordant with peripheral blood eosinophils, which did not differ significantly between groups. Multilinear regression analysis predicting FEV1% showed that AF sensitization and sputum neutrophil count were the most important predictors of FEV1 [p= 0.016 for both], followed by positive sputum culture for AF and sputum eosinophil count [p= 0.024 and 0.046 respectively]. [p = 0.105]. AF detection in sputum is associated with AF-sensitization, neutrophilic airway inflammation, and reduced lung function. This supports the concept that development of fixed airflow obstruction in asthma is consequent upon the damaging effects of airway colonization with AF

[Survey of airborne fungi spores in Qazvin [Mar-Jun 2007]]

Fungal air Spores can play a significant role in several allergic manifestations. Respiratory allergy to environmental molds is relatively common. Allergy to airborne fungi can cause rhinitis and severe asthma. The aim of this study was to investigate airborne fungal spores at Qazvin. This descriptive study was conducted by Petri-dish trapping technique. 25 different locations selected. The data were analyzed by the chi-square and t-tests. Totally, 2652 fungus colonies were counted from 150 Petri dish. The most common fungi were CLadosporium spp, Penicillium spp, Aspergillus spp. Alternaria spp. Our results seem to confirm that fungal air spores, because of its quantity and variety, can represent a serious problem for human health .Reducing these indoor fungi is necessary to improve the health of individuals with fungal-induced diseases like asthma

Common environmental allergenic fungi causing respiratory allergy in North of Iran

The aim of this study was to isolate and identify airborne fungi from locations in Babol city, Babolkenar forest and a beach on the Caspian Sea [all in North part of Iran] in spring and summer. Of 126 samples collected, the most clinically important isolated fungi were Mucor spp. [18.6%] in the forest, Alternaria spp. [13.4%] at the beach; and Cladophialophora spp. [15.8%], Mucor spp. [11.7%] and Alternaria spp. [10.6%] in the urban areas. Non-sporulating fungal isolates were grouped in Mycelia sterilia. The concentration of air spora in the forest location was significantly greater than the other locations [p<0.05]. In this study, the highest quantities of isolated fungi were found in the summer. The results showed that Alternaria spp., Clodophialophora spp. and Mucor spp. were the most predominant fungi isolated in the different locations. Since some allergenic fungi have been shown to play a role in the appearance of clinical signs in allergic conditions, the results of this study can be used by physicians and veterinarians for the diagnosis and treatment of allergies

Reactividad alérgica a Anisakis simplex y su asociación con asma bronquial en niños escolares del estado Nueva Esparta, Venezuela / Anisakis simplex allergic reactivity and its association with bronchial asthma in scholar children from Nueva Esparta state, Venezuela

La reactividad alérgica frente a Anisakis simplex se ha convertido en un problema de salud pública en muchos países del mundo. En Venezuela, se ha reportado la presencia de mamíferos marinos infectados y se ha evidenciado la presencia del parásito en pescados de consumo masivo procedentes de las zonas costeras de oriente y occidente del país. No obstante, la importancia de este helminto en el desarrollo y severidad de patologías alérgicas no ha sido evaluada en el país. El objetivo de este estudio fue evaluar la reactividad alérgica al An. simplex en un grupo de 144 niños del estado de Nueva Esparta, así como su posible relación con la severidad de asma bronquial y rinitis alérgica. Todos los niños fueron evaluados utilizando un cuestionario socioeconómico validado y modificado de Graffar y un cuestionario validado según los criterios de ARIA (International Consensus Report) para rinitis alérgica, GINA (WHO/NIH Global Initiative for Asthma) para asma bronquial y Hannifin para dermatitis atópica, respectivamente. Se realizaron encuestas para evaluar las principales fuentes de alergenos a los cuales se expone la población de estudio. Se realizó la evaluación física de los niños y se practicaron pruebas cutáneas, así como pruebas de funcionalismo pulmonar. Encontramos un alto porcentaje (45%) de niños con pruebas de piel positiva al An. simplex. Se observó una mayor prevalencia y severidad, de asma y rinitis en aquellos niños con pruebas de piel positivas a An. simplex. Dado que ha sido previamente reportada la reactividad cruzada entre Ascaris lumbricoides y An. simplex, es importante considerar que la alta reactividad encontrada frente al An. simplex pueda deberse a la co-infección por Ascaris. De igual forma, la sensibilización frente a An. simplex, ya sea especifica o por reactividad cruzada existe, lo que sugiere que las autoridades locales deben considerar ciertas medidas preventivas, para evitar el consumo de pescados crudos o posiblemente parasitados con este helminto, que podrían exacerbar la patología respiratoria.

Allergic reactivity to Anisakis simplex has become in a health problem in many parts of the World. In Venezuelan coasts, it has been found infecting marine mammals and also different parasites fishes commonly consumed by human. However, the association of this helminth with the development and severity of allergic reactivity has not been previously evaluated in Venezuela. The aim of this study was to evaluate the allergic reactivity to An. simplex in a group 144 children from Nueva Esparta, state, and its possible relation with bronquial asthma and rhinitis allergic severity. All children were evaluated using a validated socioeconomic questionnaire modified by Graffar, and a validate questionnaire according to ARIA (International Consensus Report) for allergic rhinitis, GINA (WHO/NIH Global Initiative for Asthma) for bronquial asthma and Hannifin for atopic dermatitis. Surveys were performed in order to evaluate the main sources of allergens to which the study population exposes itself. Children physical evaluation and skin prick test were performed, as well as pulmonary function test. We found a high percentage (45%) of children with positive skin prick test to An. simplex. Moreover, a greatest prevalence and severity of asthma and rhinitis, was observed in those children with positive skin prick test to An. simplex. Since it has been reported the presence of the crossed reactivity between Ascaris lumbricoides and An. simplex, it’s important to consider that the high reactivity found to An. simplex might be due to the Co-infection with A. lumbricoides. Nevertheless, the sensibilization to An. simplex, due by specific or by crossed reactivity exists, and it should be considered by local health authorities in order to prevent the exacerbation of the respiratory pathologies mediated by the consumption of fresh fishes potentially infected.

Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community-acquired pneumonias

This manuscript reviewed the literature on infection by Mycoplasma pneumoniae with emphasis on etiological aspects of childhood community-acquired pneumonias. Bibliographical research was carried out from Pubmed Medline, MDConsult, HighWire, LILACS, and direct research over the past 10 years with the following keywords: Mycoplasma pneumoniae, pneumonia, and childhood. Fifty-four articles were selected. Mycoplasma pneumoniae has a high incidence in childhood. Clinical presentation includes respiratory and extrarespiratory symptoms. Mycoplasma pneumoniae lung infection can be confused with viral or bacterial pneumonia and is unresponsive to beta-lactams. In addition, co-infections have been reported. Mycoplasma pneumoniae infection occurs in all age groups, being less frequent and more severe in children under the age of five. Its incidence as a causal agent is high. Mycoplasma pneumoniae infections constitute 20 percent-40 percent of all community-acquired pneumonias; the severity is highly variable, and this condition may lead to severe sequelae. Mycoplasma pneumoniae frequency is underestimated in clinical practice because of the lack of specific features and a diagnosis that needs serology or PCR. Effective management of M. pneumoniae infections can usually be achieved with macrolides. In Brazil, epidemiological studies are needed in order to assess the incidence of this bacterium.

Seroprevalence of Chlamydia pneumoniae in asthmatic children from Northern India.

Serum from children with chronic persistent asthma was subjected to C. pneumoniae IgG antibody determination. C. pneumoniae IgG serology suggestive of persistent infection was significantly higher in chronic persistent asthma group than in the control group. Seropositivity was significantly more in moderate and severe persistent groups than in the control subjects. There was no evidence of acute C. pneumoniae infection (IgM serology done in duplicate) in acute exacerbations.

Seroprevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in Stable Asthma and Chronic Obstructive Pulmonary Disease

Mycoplasma pneumoniae and Chlamydia pneumoniae have been suggested to take part in the acute exacerbation of bronchial asthma and chronic obstructive pulmonary disease (COPD). Several studies have questioned whether they may play pathogenic roles in connection with bronchial asthma and COPD. This study was designed to evaluate the seroprevalences of M. pneumoniae and C. pneumoniae in stable asthma and COPD patients, and to compare with control patients. The medical records of one hundred forty patients who underwent M. pneumoniae and C. pneumoniae serology were retrospectively reviewed. Seroprevalences of M. pneumoniae and C. pneumoniae in the asthma group (11.1% and 8.3%, respectively) were higher than in the control group (4.4% and 2.2%, respectively) without statistical significance. The seroprevalence of M. pneumoniae in the COPD group (16.9%) was significantly higher than in the control group, and the seroprevalence of C. pneumoniae in the COPD group (3.4%) was higher than in the control group without statistical significance. This study raises important questions about the relation of M. pneumoniae and C. pneumoniae infection with stable asthma or COPD.

Flora bacteriana orofaríngea en sujetos asmáticos y sanos / Oropharinx bacterial flora in healty and asthmatic patients

Antecedntes: En la orofaringe coexisten, en menor o mayor grado, bacterias que juegan un papel decisivo en la manifestación de enfermedad, incluso en el asma. Material y método: Se realizó cultivo de exudado faríngeo a 248 sujetos, 128 asmáticos y 120 aparentemente sanos. Del grupo de los asmáticos 55.46 por ciento fueron de sexo masculino y 44.54 por ciento del femenino, 72.9 por ciento tuvieron más de dos cuadros de asma por mes. Resultados: En el cultivo de 107 asmáticos se reporta la existencia de gérmenes como estafilococo dorado coagulasa positivo 43.56 por ciento (p = NS), el estafilococo beta-hemolítico (72.97 por ciento (p = 0.001) Candida albicans 70.58 por ciento (p = 0.004), estafilococo alfa hemolítico 61.42 por ciento (p = 0.008). Conclusiones: Cuando existen más de dos gérmenes en un mismo individuo, frecuentemente se detecta Staphilococcus aureus coagulasa positivo con el Streptococcus beta hemoliticus 42.10 por ciento o con Candida albicans (31.567 por ciento. Se propone dimensionar el problema al que se enfrenta para ordernar un medicamento o examen de laboratorio con oportunidad y cuando se espera obtener el óptimo beneficio para la salud de la población

Chlamydia pneumoniae, enfermedades cardiovasculares y asma / Chlamydia pneumoniae, cardiovascular diseases and asthma

Como patógeno respiratorio, C. pneumoniae ha sido asociada con crisis de asma bronquial como uno de varios factores desencadenantes. Las evidencias seroepidemiológicas, histopatológicas y de biología molecular que relacionan además la infección por C. penumonaie con la producción de angiopatía coronaria van en aumento. Se revisan los antecedentes que han permitido encontrar esta relación, incluyendo nuestra propia experiencia, aunque se desconoce el grado de responsabilidad atribuible a la infección en esta afección degenerativa

Repercusión de las infecciones respiratorias en el asma y procedimientos terapéuticos / Repercution of the breathing infections in asthma and terapeutic proceedings

Con el propósito de evaluar la importancia de las infecciones respiratorias en el asma se hace una revisión epidemiológica de las infecciones respiratorias virales que afectan más a los niños y de las bacterianas con un menor grado de afección. Se hace una revisión de las sinusitis que tienen mayor repercusión en los adultos; además, se revisa la implicación que puede tener Chlamydia pneumoniae en niños y adultos y la demostración de que ésta exacerba los cuadros de asma. Por otro lado, se presentan las aplicaciones terapéuticas para controlar la hiperreactividad bronquial relacionada con estas infecciones que provocan asma

Helicobacter pylori infection associated with gastroesophageal reflux in patients with bronchial asthma: effect of eradication

This study explored the effect of Helicobacter pylori [Hp] eradication on chronic asthma patients with GERD associated with Hp infection. Forty-five adult patients with chronic moderate to severe asthma and GERD associated Hp infection were randomly assigned to three groups. Group I [triple therapy group] [n = 15] received 1000 mg amoxicillin bid and clarithromycin 500 mg bid for two weeks and omeprazole 20 mg bid for three months, Group II [omeprazole group] [n = 15] received omeprazole 20 mg bid for three months and Group III [control] [n = 15] received placebo for three months. Observation continued for three months after drug trial phase [wash out phase]. It was concluded that Hp has significant role in chronic asthma patients associated with GERD and its eradication results in long term improvement of both asthma and GERD

Búsqueda de Mycoplasma pneumoniae, M. hominis y M. fermentans en infecciones respiratorias / Searching for Mycoplasma pneumonia, Mycoplasma hominis, and Mycoplasma fermentans in respiratory infections

Mycoplasma pneumoniae es un microorganismo que produce un amplio espectro de síntomas respiratorios y otros síndromes clínicos. La neumonía micoplásmica es extremadamente variada, su inicio es de dos semanas después de la exposición y generalmente es gradual, con dolor de cabeza, fiebre, escalofrío, eritema de las membranas timpánicas y de la mucosa de la faringe posterior; en la mayoría de los casos se acompaña de tos no productiva. La imagern radiológica de la neumonía causada por esta bacteria no es característica, por lo que no se puede diferenciar de la originada por virus. La metodología utilizada en este trabajo se estandarizó con cepas de referencia, utilizando pruebas bioquímicas, fisiológicas y serológicas. Se estudiaron 157 muestras respiratorias de 145 enfermos. La neumonía y/o bronconeumonía fueron los diagnósticos clínicos en donde se encontró a Mycoplasma sp. con mayor frecuencia (46.2 por ciento), seguidas por neumonía complicada (20 por ciento). En total se obtuvieron 40 cepas de Mycoplasma sp.; de éstas correspondió a M. pneumoniae 13.1 por ciento, M. Hominis 9.6 por ciento y M. fermentans 4.8 por ciento. Se analizaron 60 sueros por la prueba de inhibición metabólica para determinar anticuerpos contra Mycoplasma sp.; ello permitió detectar a pacientes con títulos significativos de anticuerpos mayores de 1:32 que tuvieron cultivo negativo; sin esta prueba no se hubiera sospechado la presencia de la bacteria en el paciente; esto demuestra que, para obtener un diagnóstico más preciso, se requiere por lo menos del cultivo y la serología

Role of fungi in allergic bronchitis

Sputum samples from thirty asthmatic patients with recurrent infections were examined for fungi, as well as for acid fast bacilli and other pathogenic bacteria. 60 of cases were positive for fungi; either alone or associated with other bacteria. No one case showed acid fast bacilli. Eosinophilic count and serum immunoglobulin E [IgE] level were significantly high in all cases. Most of the patients, especially those infected with Candida species, showed clinical improvement after antimycotic treatment. It was concluded that hypersensitivity to fungal antigens was accused in many cases of allergic bronchitis. So the role of fungi in such cases must be taken in consideration and should be thoroughly investigated in order to choose the proper line of treatment

Role of aspergillus species in the pathogenesis of bronchial asthma in paediatrics

As understanding of the inflammatory nature of asthma increases, so does our ability to treat asthma. The fine tuned management of asthma requires awareness and the ability to control these other factors. Allergic bronchopulmonary aspergillosis is not just an associated condition that may make asthma worse but it is also a complication of asthma and can be confused with asthma. This study included 25 asthmatic children who suffer sever interactable asthma, their ages ranged from 4-13 years [mean=8.0 +/- 3.5]. They were fourteen males [56%] and eleven females [44%]. All gave [+ve] skin test to aspergillus mix extract. Their total Ig[E] was very high with mean value [505.2] I. U. /ml] which ranged from [44 to 8950 I.U./ ml]; and absolute eosinophllic count mean value was [573 +/- 83/ml]. The atopic ones were 76% and 80% gave positive family history of allergy. Their X-Ray findings showed heavy infilterates and tendancy to central destruction in most of them. While their course of the disease was very progressive and interactable, they only improved on corticosteroid therapy. The specific Ig[E] against Aspergillus fumigatus gave [-ve] results. We found positive cultures for Fungi in Sputum of 25 asthmatics, they were Aspergillus niger [52%], Aspergillus flavus [12%], Aspergillus fumigtus [4%], Candida [36%] and Penicillium [24%] while 20% showed no growth. While the 10 normal controls showed Asp. niger [20%], Candida [40%], Penicillium [10%], and the rest [30%], showed no growth. We conclude that Aspergillosis could affect asthmatic children as well as adults and needs full accurate investigation as well as steroids therapy; when suspicious interactable course noticed in them